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Research

Tissue Microarray (TMA)

 

Tissue Microarray (TMA) is a powerful tool for cancer research and drug discovery. In the TMA technique, individual tissue cores are obtained with a hollow needle from different formalin-fixed, paraffin-embedded tissue blocks and are then inserted in a recipient paraffin block in a precisely spaced, array pattern. Each TMA block can be sectioned using a microtome. All resulting TMA slides have the same tissues in the same coordinate positions. Each microarray block can be cut into ~35 sections. These collections of tissue cores results in a dramatic increase in throughput for in situ examination of gene status and gene expression from archival specimens. 

Applications commonly employed are immunohistochemistry, conventional staining, in situ Hybridization (ISH), fluorescent in situ hybridization (FISH), RNA or DNA expression analysis, evaluation of various biomarkers, TUNEL assay for apoptosis and other morphological and clinical characterization of different tissues. 

Take advantage by saving reagent costs while maintaining uniformity of assays. Development of TMAs requires significant interaction between the constructor and investigator.

Types of TMA

There are two types of TMA available. Please contact us to get your own design.

  • Paraffin TMA: TMA constructed from paraffin blocks of tissue or cell blocks using 1 mm or 2 mm cores. Additional paraffin core storage -- we offer to separately store (in an Eppendof tube) an additional paraffin core obtained adjacent to the TMA target for future molecular biology study.
  • Frozen TMA: Frozen TMA blocks in OCT can be constructed from frozen tissue blocks or frozen tissue fragments.

Contact & Links

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Contact Information

Muralidharan Jayaraman, PhD
Director of Research Core Operations
P: (405) 271-6890
E: muralidharan-jayaraman@ouhsc.edu

Core Hours

Monday through Friday
8 am to 5 pm

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Usage Acknowledgement

If research supported by the Stephenson Cancer Center core facilities results in a publication or news release, please acknowledge this support in your manuscript. Following publication, please send us one of your reprints for our records.