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Research

Histology and Immunohistochemistry

 

Histology Services

Services include:

  • Decalcification with acid-based solution or neutral EDTA-based solution that preserve DNA integrity
  • Sucrose cryoprotection of fixed tissue
  • Freezing tissue into OCT blocks
  • Processing tissue blocks or culture cells into formalin-fixed, paraffin-embedded tissue blocks
  • Frozen sections at routine thickness (5 μm) or at a specific thickness (minimal thickness is 2 μm)
  • Paraffin sections at routine thickness (5 μm) or at a specific thickness, paraffin rolls at a specific thickness (minimal thickness is 2 μm)
  • Hematoxylin and eosin (HE) stain
  • Conventional special stain [Click here for the list]
  • Enzyme histochemistry [Click here for the list]

Immunohistochemistry Services

Services include:

  • Automated peroxidase-Diaminobenzidine (PO-DAB) or Alkaline phosphatase-variable chromogen (AK-VC) based single stain immunohistochemistry, paraffin block or frozen block, with hematoxylin counter stain
  • Automated double immunohistochemistry with PO-DAB/AK-VC combined staining, with hematoxylin counter stain, with and without subtraction
  • Automated PO-DAB immunohistochemistry with clinically-used antibodies through the Department of Pathology [Click here for the list]
  • Manual immiunohistochemistry with or without combination with conventional stains or other techniques
  • Titration of antibody and/or testing of antigen retrieval protocols for paraffin embedded or frozen tissue with PO-DAB technique

Sample Images

Conventional and double staining examples

Contact & Links

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Contact Information

Muralidharan Jayaraman, PhD
Director of Research Core Operations
P: (405) 271-6890
E: muralidharan-jayaraman@ouhsc.edu

Core Hours

Monday through Friday
8 am to 5 pm

Access the Core

The Stephenson Cancer Center uses iLab for online scheduling for shared resources.

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Usage Acknowledgement

If research supported by the Stephenson Cancer Center core facilities results in a publication or news release, please acknowledge this support in your manuscript. Following publication, please send us one of your reprints for our records.